Recombinant Human UBE2B, His-tagged UBE2B-55H

Recombinant Human UBE2B, His-tagged

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Recombinant Human UBE2B, His-tagged

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Cat. No. : UBE2B-55H
Product Overview : Recombinant human UBE2B protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 21.4 kDa (188aa).
Description : UBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on "Lys-164". It is required for postreplication repair of UV-damaged DNA. It may be involved in neurite outgrowth.
Source : E.coli.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
Purity : > 90% by SDS – PAGE.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS.
Storage : can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1jas. :

Gene Name : UBE2B ubiquitin-conjugating enzyme E2B (RAD6 homolog) [ Homo sapiens ]
Synonyms : UBE2B; ubiquitin-conjugating enzyme E2B (RAD6 homolog); HR6B; UBC2; HHR6B; RAD6B; E2-17kDa; ubiquitin-conjugating enzyme E2B; E2 protein; ubiquitin-protein ligase B; ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2 B; EC 6.3.2.19; Ubiquitin-protein ligase B; Ubiquitin carrier protein B; hHR6B
Gene ID : 7320
mRNA Refseq : NM_003337
Protein Refseq : NP_003328
MIM : 179095
UniProt ID : P63146
Chromosome Location : 5q31.1
Pathway : Ubiquitin mediated proteolysis
Function : ATP binding; ligase activity; nucleotide binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity

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