"UBE2B" Related Products

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Recombinant Human UBE2B, His-tagged

Cat. No.: UBE2B-55H
Product Overview: Recombinant human UBE2B protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.MW = 21.4 kDa (188aa).
Description: UBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on "Lys-164". It is required for postreplication repair of UV-damaged DNA. It may be involved in neurite outgrowth.
Source: E.coli.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
Purity: > 90% by SDS – PAGE.
Concentration: 0.5 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS.
Storage: can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: UBE2B ubiquitin-conjugating enzyme E2B (RAD6 homolog) [ Homo sapiens ]
Synonyms: UBE2B; ubiquitin-conjugating enzyme E2B (RAD6 homolog); HR6B; UBC2; HHR6B; RAD6B; E2-17kDa; ubiquitin-conjugating enzyme E2B; E2 protein; ubiquitin-protein ligase B; ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2 B; EC 6.3.2.19; Ubiquitin-protein ligase B; Ubiquitin carrier protein B; hHR6B
Gene ID: 7320
mRNA Refseq: NM_003337
Protein Refseq: NP_003328
MIM: 179095
UniProt ID: P63146
Chromosome Location: 5q31.1
Pathway: Ubiquitin mediated proteolysis
Function: ATP binding; ligase activity; nucleotide binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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