Dihydrofolate Reductase Inhibitor Screening Kit

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Dihydrofolate Reductase Inhibitor Screening Kit

DHFR Related Products

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Cat.No. : Kit-2100
Product Overview : Dihydrofolate Reductase Inhibitor Screening Kit is designed for screening DHFR inhibitors. MTX is used as a positive control. The DHFR activity is monitored by the reduction in absorbance reading at OD340 nm, while potential inhibitors arrest this decrease. The kit is adapted to a 96-well format and provides a rapid, simple, sensitive, and reliable test for high-throughput screening of DHFR inhibitors.
Size : 100 assays
Applications : Screening/characterizing/studying ACE2 inhibitors/activators
Storage : Upon receiving the kit, store DHFR Substrate at -80°C. Store other components at -20°C. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay. Upon opening, use within two months.DHFR Assay Buffer: Warm to room temperature before use. Store at 4°C or -20°C.DHFR Substrate: Aliquots and store at -80°C, protect from light. Avoid repeated freeze/thaw.Dihydrofolate Reductase: Store at –20°C. Avoid repeated freeze/thaw. Keep on ice while in use.NADPH: Reconstitute with 200 μl DHFR Assay Buffer to generate NADPH Stock Solution. Aliquot and store at –20°C. Keep on ice while in use.Methotrexate: Aliquot and store at -20°C. Protect from light. Keep at room temperature while in use.

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