- Mouse(1)
ES-D3 (mouse pluripotent embryonic stem cell) whole cell lysate
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Cat.No. : | ES-D3-573M Optional Service: Optional requirements on this protein |
Species : | Mouse |
Tissue Type : | Embryonic |
Preparation method : | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
Size : | 50 ug |
Recommended Usage : | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Storage Buffer : | In modified RIPA Lysis Buffer. |
Applications : | Western Blot;Immunoprecipitation; |
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