Name: ES-D3 (mouse pluripotent embryonic stem cell) whole cell lysate
SKU: ES-D3-573M

Species :	Mouse
Tag :	Non
Tissue Type :	Embryonic
Preparation method :	Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Size :	50 ug
Recommended Usage :	Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer :	In modified RIPA Lysis Buffer.
Applications :	Western Blot;Immunoprecipitation;