GMP Recombinant Streptococcus pyogenes Cas9 protein(Asp2-Asp1368)

• Cat.No.: Cas9-21S
• Product Overview: Recombinant Streptococcus pyogenes Cas9(Asp2-Asp1368)(Q99ZW2) was expressed in E. coli, in an animal component free process under cGMP guidelines.

Specification

• Species: Streptococcus pyogenes
• Source: E.coli
• Tag: Non
• Protein Length: 2-1368 aa
• Form: Lyophilized from 30 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4.
• Bio-activity: Three batches of Cas9 Nuclease cleaved substrate DNA standards in vitro. Results show that the cleavage activity are similar across all batches, indicating stable activity across batches.
• Molecular Mass: The protein has a predicted MW of 163 kDa.
• Endotoxin: ≤10 EU/mg
• Purity: ≥95% by SEC-HPLC; ≥95% by RP-HPLC
• Storage: Store at -20 ± 5°C. Avoid repeat freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: Cas9
• Synonyms: Cas9