"Protease" Related Products

HIV-1 Protease Inhibitor Screening Kit

Cat.No.: Kit-2143
Product Overview: HIV-1 Protease Inhibitor Screening kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate resulting in the release of free fluorophore, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a HIV-1 Protease inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease.
Size: 100 assays
Description: Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV-1 protease, HIV virions remain non-infectious.
Applications: Screening/studying/characterizing inhibitors of HIV-1 Protease
Storage: Store kit at -80°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the experiment.HIV-1 Protease Assay Buffer and Dilution Buffer: Bring to room temperature before use. Store at -20°C.HIV-1 Protease: To each vial of HIV1 Protease, add 70 µl of HIV Protease Dilution Buffer as needed. Aliquot and store at -80°C. Avoid repeated freeze/thaw. Use within two months.
Kit Components: HIV-1 Protease Assay Buffer: 25 mlHIV-1 Protease Dilution Buffer: 1 mlHIV-1 Protease Substrate: 0.2 mlHIV-1 Protease: 2 VialsHIV-1 Protease Inhibitor (1 mM Pepstatin A in DMSO): 20 μl
Detection method: Fluorescence (Ex/Em = 330/450 nm)
Compatible Sample Types: Variable: Inhibitors, small molecules, proteins, etc.
Features & Benefits: • Simple and High throughput adaptable• Detect using a fluorometer or fluorescence microplate reader.

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.