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Specification Related Products

Cat.No. :	Kit-2143
Product Overview :	HIV-1 Protease Inhibitor Screening kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate resulting in the release of free fluorophore, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a HIV-1 Protease inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease.
Size :	100 assays
Applications :	Screening/studying/characterizing inhibitors of HIV-1 Protease
Storage :	Store kit at -80°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read the entire protocol before performing the experiment.HIV-1 Protease Assay Buffer and Dilution Buffer: Bring to room temperature before use. Store at -20°C.HIV-1 Protease: To each vial of HIV1 Protease, add 70 µl of HIV Protease Dilution Buffer as needed. Aliquot and store at -80°C. Avoid repeated freeze/thaw. Use within two months.