"hROS" Related Products

      hROS Detection Kit

      Cat.No. : Kit-0467
      Product Overview : A selective indicator for the detection of Highly Reactive Oxygen Species, hROS.
      Description : A new novel probe, Aminophenyl fluorescein (APF), developed by Tetsuo Nagano et. al. (1), is a general selective indcator for the detection of highly Reactive Oxygen Species (hROS). The probe has little reactivity towards other ROS such as: singlet oxygen (O21), superoxide (O2-•), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1. APF is a cell permeable indicator that can be used to detect Hydroxyl Radical (.OH), Peroxynitrite: (ONOO-) and hypochlorite (-OCl) production in cells.Several fluorescent probes such as 2",7"-dichlorodihydrofluorescein (DCFH) and di-hydrorhodamine123, have been developed to detect Reactive Oxygen Species (ROS). As Hempel and co-workers have indicated, DCFH and di-hydrorhodamine123 can react with various ROS and oxidizing species such as hydrogen peroxide, hypochlorite, superoxide, nitric oxide, ferrous ion, and others. These probes should be considered as detecting a broad range of ROS.
      Applications : Flow Cytometer, Fluorescence Microscope, Fluorescence plate reader
      Usage : 1. For Research use only. Not for use in diagnostic procedures.2. Practice safe laboratory procedures by wearing protective clothing and eyewear.
      Storage : 1. Long Term Storage: Store contents as labeled.2. Upon Arrival: 2-8°C.3. Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) should be stored at 4-80C. Protect from light until ready to use. The diluted material must be used immediately and discard any unused diluted material.
      Kit Components : One vial of APF
      Features & Benefits : 1. Quenched Cell permeable dye.2. One Step, No wash Homogenous assay.3. Adaptable to High throughput assay platforms.4. Can monitor multiple time points to follow real time kinetics.5. Non-destructive cell based assay allows monitoring of additional parameters.6. Applications-Fluorescent plate reader/Flow Cytometery/Fluorescent Microscopy.

      Online Inquiry

      Note: There will be extra charge for optional service!

      Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

      Optional requirements on this protein    +Expand

      Price Inquiry

      Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.