Human Adrenal Membrane Lysate
Cat.No. : | Adrenal-13H |
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Species : | Human |
Source : | Human Adrenal |
Tag : | Non |
Concentration : | 2 mg/mL or better |
Tissue Type : | Adrenal |
Shipping : | Dry ice |
Preparation method : | Human adrenal tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human adrenal tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated adrenal tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated adrenal tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Diagnosis : | Normal |
Storage Buffer : | HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40. |
Storage Instruction : | Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt. |
Applications : | Adrenal membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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Not For Human Consumption!
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