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Human Breast Adenocarcinoma MCF7 Whole Cell Lysate

Cat.No. : MCF7-001WCY
Product Overview : MCF7 (Human Breast Adenocarcinoma) Whole Cell Lysate
  • Specification
  • Gene Information
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Description : MCF-7 is a human breast cancer cell line which was first isolated in 1970 from the malignant adenocarcinoma breast tissue of a 69-year old woman. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells can carry B or C type retrovirus and are thought to represent a category 2 pathogen (P2 containment). Cells express the wild-type and variant estrogen receptors as well as progesterone receptor.
Source : Breast
Species : Human
Applications : IP, WB
Stability : The lysate is stable for at least one year from date of shipping when stored at -80 centigrade. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20 centigrade for long term storage.
Storage : Store the lysate at -80 centigrade and avoid repeated freeze / thaw cycles.
Concentration : 1 vial of 100 μg whole cell lysate in RIPA buffer.
1 vial of 250 μL 2 x SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH 6.8, 10 % Glycerol, 0.002 % Bromphenol blue, 100 mM DTT)
Storage Buffer : Modified RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma) and 1 mM Na3VO4)
Shipping : Shipped on dry ice.

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◆ Lysates
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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