Human DDX5 Knockdown Cell Lysate

• Cat.No.: DDX5-134HKCL
• Product Overview: WB-validated DDX5 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 69 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg DDX5 KD HeLa cell lysate
• Protein Pathways: Spliceosome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: DDX5 DEAD (Asp-Glu-Ala-Asp) box helicase 5 [ Homo sapiens (human) ]
• Official Symbol: DDX5
• Synonyms: DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD), G17P1, HLR1; probable ATP-dependent RNA helicase DDX5; p68; DEAD box-5; RNA helicase p68; DEAD box protein 5; ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); HLR1; G17P1; HUMP68; DKFZp434E109; DKFZp686J01190
• Gene ID: 1655
• mRNA Refseq: NM_004396
• Protein Refseq: NP_004387
• MIM: 180630
• UniProt ID: P17844