Human FASN Knockdown Cell Lysate

• Cat.No.: FASN-344HKCL
• Product Overview: WB-validated FASN Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 273 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg FASN KD HeLa cell lysate
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: FASN fatty acid synthase [ Homo sapiens (human) ]
• Official Symbol: FASN
• Synonyms: FASN; fatty acid synthase; FAS; SDR27X1; short chain dehydrogenase/reductase family 27X; member 1; short chain dehydrogenase/reductase family 27X, member 1; OA-519; MGC14367; MGC15706
• Gene ID: 2194
• mRNA Refseq: NM_004104
• Protein Refseq: NP_004095
• MIM: 600212
• UniProt ID: P49327