Human Hexokinase II (HKII) Inhibitor Screening kit uses the HKII"s ability to convert glucose into glucose-6-phosphate. Glucose-6-phosphate is oxidized by glucose-6- phosphate dehydrogenase to form NADH, which reduces a probe that shows strong absorbance at 450 nm. In the presence of Bromopyruvic Acid, reactions are impeded, thus decreasing the rate and/or extent of generation of Hexokinase -dependent absorbance at OD 450 nm. Hexokinase Inhibitor Control is included to compare the efficacy of test inhibitors. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of Hexokinase. The assay is high-throughput adaptable and can be performed in less than 30 min.
Size :
100 assays
Applications :
Screening/characterizing/studying potential inhibitors of human Hexokinase
Storage :
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.HK Assay Buffer: Bring to room temperature before use. Store at -20 °C or 4°C.HK Coenzyme and HK Developer: Reconstitute each with 220 µl dH2O. Pipette up and down to dissolve completely. Store at -20°C. Use within two months. Keep on ice while in use.HK Converter: Reconstitute with 220 µl HK Assay Buffer. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Usewithin two months.Hexokinase II: Ready to use. Store at -20°C.HK Inhibitor Control: Reconstitute with 100 µl dH2O. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.