Human Hexokinase Inhibitor Screening Kit

• Cat.No.: Kit-2111
• Product Overview: Human Hexokinase II (HKII) Inhibitor Screening kit uses the HKII"s ability to convert glucose into glucose-6-phosphate. Glucose-6-phosphate is oxidized by glucose-6- phosphate dehydrogenase to form NADH, which reduces a probe that shows strong absorbance at 450 nm. In the presence of Bromopyruvic Acid, reactions are impeded, thus decreasing the rate and/or extent of generation of Hexokinase -dependent absorbance at OD 450 nm. Hexokinase Inhibitor Control is included to compare the efficacy of test inhibitors. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of Hexokinase. The assay is high-throughput adaptable and can be performed in less than 30 min.

Specification

• Size: 100 assays
• Applications: Screening/characterizing/studying potential inhibitors of human Hexokinase
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.HK Assay Buffer: Bring to room temperature before use. Store at -20 °C or 4°C.HK Coenzyme and HK Developer: Reconstitute each with 220 µl dH2O. Pipette up and down to dissolve completely. Store at -20°C. Use within two months. Keep on ice while in use.HK Converter: Reconstitute with 220 µl HK Assay Buffer. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Usewithin two months.Hexokinase II: Ready to use. Store at -20°C.HK Inhibitor Control: Reconstitute with 100 µl dH2O. Aliquot and store at -20°C. Avoid freeze/thaw. Keep on ice while in use. Use within two months.