Human NHERF1 Knockdown Cell Lysate

• Cat.No.: NHERF1-377HKCL
• Product Overview: WB-validated NHERF1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 39 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg NHERF1 KD HeLa cell lysate
• Protein Families: Druggable Genome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: NHERF1 NHERF family PDZ scaffold protein 1 [ Homo sapiens (human) ]
• Official Symbol: NHERF1
• Synonyms: NHERF1; NHERF family PDZ scaffold protein 1; EBP50; NHERF; NHE-RF; NHERF-1; NPHLOP2; SLC9A3R1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor 1; Na+/H+ exchange regulatory co-factor; SLC9A3 regulator 1; ezrin-radixin-moesin binding phosphoprotein-50; regulatory cofactor of Na(+)/H(+) exchanger; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• Gene ID: 9368
• mRNA Refseq: NM_004252
• Protein Refseq: NP_004243
• MIM: 604990
• UniProt ID: O14745