Human PAFAH1B1 Knockdown Cell Lysate

• Cat.No.: PAFAH1B1-259HKCL
• Product Overview: WB-validated PAFAH1B1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 47 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg PAFAH1B1 KD HeLa cell lysate
• Protein Pathways: Ether lipid metabolism, Metabolic pathways
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: PAFAH1B1 platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa) [ Homo sapiens (human) ]
• Official Symbol: PAFAH1B1
• Synonyms: PAFAH1B1; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); MDCR, MDS, Miller Dieker syndrome chromosome region, platelet activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, platelet activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa); platelet-activating factor acetylhydrolase IB subunit alpha; LIS1; PAFAH; LIS-1; PAFAH alpha; PAF-AH alpha; PAF-AH 45 kDa subunit; lissencephaly 1 protein; lissencephaly-1 protein; PAF acetylhydrolase 45 kDa subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa); platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD); MDS; LIS2; MDCR
• Gene ID: 5048
• mRNA Refseq: NM_000430
• Protein Refseq: NP_000421
• MIM: 601545
• UniProt ID: P43034