Human Pons Tissue Lysate

• Cat.No.: Brain: Pons-47H

Specification

• Species: Human
• Tag: Non
• Form: The human pons tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
• Concentration: 3 mg/ml
• Tissue Type: Brain: Pons
• Preparation method: Human pons tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pons tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pons tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pons tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.
• Safety Testing: This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
• Diagnosis: Normal
• Expiration Date: One half year from date of receipt
• Size: 0.1 mg, 0.5 mg
• Storage Instruction: This lysate should be aliquoted in working amounts and may be stored for up to one month at 2-8°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
• Applications: This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.