- Human(1)
Human Pons Tissue Lysate
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Cat.No. : | Brain: Pons-47H Optional Service: Optional requirements on this protein |
Species : | Human |
Form : | The human pons tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. |
Concentration : | 3 mg/ml |
Tissue Type : | Brain: Pons |
Preparation method : | Human pons tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pons tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pons tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pons tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Safety Testing : | This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request. |
Diagnosis : | Normal |
Expiration Date : | One half year from date of receipt |
Size : | 0.1 mg, 0.5 mg |
Storage Instruction : | This lysate should be aliquoted in working amounts and may be stored for up to one month at 2-8°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C. |
Applications : | This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
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