Human PSMB10 Knockdown Cell Lysate

• Cat.No.: PSMB10-282HKCL
• Product Overview: WB-validated PSMB10 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 29 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg PSMB10 KD HeLa cell lysate
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: PSMB10 proteasome (prosome, macropain) subunit, beta type, 10 [ Homo sapiens (human) ]
• Official Symbol: PSMB10
• Synonyms: PSMB10; proteasome (prosome, macropain) subunit, beta type, 10; MECL1; proteasome subunit beta type-10; beta2i; LMP10; MGC1665; proteasome MECl-1; macropain subunit MECl-1; proteasome subunit MECL1; proteasome subunit beta 7i; proteasome subunit beta-2i; low molecular mass protein 10; proteasome catalytic subunit 2i; multicatalytic endopeptidase complex subunit MECl-1; FLJ00366
• Gene ID: 5699
• mRNA Refseq: NM_002801
• Protein Refseq: NP_002792
• MIM: 176847
• UniProt ID: P40306