Human RPN1 Knockdown Cell Lysate

• Cat.No.: RPN1-421HKCL
• Product Overview: WB-validated RPN1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 69 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg RPN1 KD HeLa cell lysate
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: RPN1 ribophorin I [ Homo sapiens (human) ]
• Official Symbol: RPN1
• Synonyms: RPN1; ribophorin I; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; oligosaccharyltransferase 1 homolog (S. cerevisiae); OST1; RPN-I; ribophorin-1; oligosaccharyltransferase 1 homolog; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; RBPH1; DKFZp686B16177
• Gene ID: 6184
• mRNA Refseq: NM_002950
• Protein Refseq: NP_002941
• MIM: 180470
• UniProt ID: P04843