Human SMARCE1 Knockdown Cell Lysate

• Cat.No.: SMARCE1-297HKCL
• Product Overview: WB-validated SMARCE1 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 47 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg SMARCE1 KD HeLa cell lysate
• Protein Families: Transcription Factors
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: SMARCE1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 [ Homo sapiens (human) ]
• Official Symbol: SMARCE1
• Synonyms: SMARCE1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BAF57; BRG1-associated factor 57; chromatin remodeling complex BRG1-associated factor 57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1; FLJ35648
• Gene ID: 6605
• mRNA Refseq: NM_003079
• Protein Refseq: NP_003070
• MIM: 603111
• UniProt ID: Q969G3