Human UBE2L3 Knockdown Cell Lysate

• Cat.No.: UBE2L3-461HKCL
• Product Overview: WB-validated UBE2L3 Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 18 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg UBE2L3 KD HeLa cell lysate
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: UBE2L3 ubiquitin-conjugating enzyme E2L 3 [ Homo sapiens (human) ]
• Official Symbol: UBE2L3
• Synonyms: UBE2L3; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme E2 L3; UBCH7; ubiquitin-protein ligase L3; ubiquitin carrier protein L3; ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme UBCH7; E2-F1; L-UBC; UbcM4
• Gene ID: 7332
• mRNA Refseq: NM_001256355
• Protein Refseq: NP_001243284
• MIM: 603721
• UniProt ID: P68036