Lactate Dehydrogenase B (LDHB) Activity Assay Kit

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Lactate Dehydrogenase B (LDHB) Activity Assay Kit

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Cat.No. : Kit-0510
Product Overview : The microplate assay is used to determine Lactate dehydrogenase B activity (LDHB) in a sample utilizing the immunocapture technique. The specific native LDHB enzyme is immune-captured within the wells of the microplate and activity is determined by following the production of NADH catalyzed by the enzyme. The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient-37000 M-1 cm-1). The immune-capture based activity assay kit contains all the required reagents for a fast and simple measurement of LDHB activity in a whole cell extract, tissue homogenate, or serum.
Applications : ELISA, WB
Storage : All components are shipped +4ºC. Reagent dye, coupler, LDHB and NAD+ are shipped lyophilized. Before use, rehydrate according to the instructions in Section 6-Reagent Preparation and vortex each tube thoroughly to dissolve. After hydration, unused amounts of these four materials should be aliquoted and stored at -20°C, avoiding additional freeze/thaw cycles. Store all other components at 4°C. This kit is Stable for 6 months from receipt.

Products Types

◆ Recombinant Protein

Ldhb-1306MRecombinant Mouse Ldhb Protein, MYC/DDK-tagged+Inquiry
LDHB-424HRecombinant Human LDHB Protein, MYC/DDK-tagged+Inquiry
LDHB-3370RRecombinant Rat LDHB Protein+Inquiry
Ldhb-1818RRecombinant Rat Ldhb protein, His-tagged+Inquiry
LDHB-9022MRecombinant Mouse LDHB Protein+Inquiry

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◆ Native Protein

LDH2-123HNative Human Lactate Dehydrogenase 2+Inquiry
LDH2-220HNative Human Lactate Dehydrogenase 2+Inquiry

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◆ Lysates

LDHB-4788HCLRecombinant Human LDHB 293 Cell Lysate+Inquiry

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LDHB

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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