Lactate Dehydrogenase B (LDHB) Activity Assay Kit

• Cat.No.: Kit-0510
• Product Overview: The microplate assay is used to determine Lactate dehydrogenase B activity (LDHB) in a sample utilizing the immunocapture technique. The specific native LDHB enzyme is immune-captured within the wells of the microplate and activity is determined by following the production of NADH catalyzed by the enzyme. The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient-37000 M-1 cm-1). The immune-capture based activity assay kit contains all the required reagents for a fast and simple measurement of LDHB activity in a whole cell extract, tissue homogenate, or serum.

Specification

• Applications: ELISA, WB
• Storage: All components are shipped +4ºC. Reagent dye, coupler, LDHB and NAD+ are shipped lyophilized. Before use, rehydrate according to the instructions in Section 6-Reagent Preparation and vortex each tube thoroughly to dissolve. After hydration, unused amounts of these four materials should be aliquoted and stored at -20°C, avoiding additional freeze/thaw cycles. Store all other components at 4°C. This kit is Stable for 6 months from receipt.

Reviews

04/27/2022

I highly endorse the utilization of the LDHB Protein for researchers seeking a superior protein solution that not only meets their experimental needs but also benefits from the exceptional technical support provided by the manufacturer.

11/05/2020

Their expertise has proven instrumental in overcoming obstacles and has greatly contributed to the success of my research endeavors.

10/14/2016

Their team of experts has been extremely helpful in addressing any concerns or queries I encountered during my experiments.

Q&As

Does LDHB play a role in heart disease?

Yes, LDHB dysregulation is associated with heart conditions like myocardial infarction (heart attack) and heart failure.

Are there specific populations more susceptible to LDHB-related conditions?

While LDHB-related diseases can affect anyone, some genetic predispositions may heighten the risk in certain ethnic groups.

How is LDHB protein clinically relevant?

It plays a role in various physiological processes and has implications in disease states.

How does LDHB contribute to cancer?

In certain cancers, elevated LDHB levels promote tumor growth by supporting the Warburg effect, where cancer cells use glycolysis even in the presence of oxygen.

Can LDHB be a therapeutic target in cancer treatment?

Targeting LDHB shows promise in inhibiting cancer growth, and there's ongoing research in developing drugs that target this enzyme.