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Lactate Dehydrogenase B (LDHB) Activity Assay Kit
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Cat.No. : | Kit-0510 |
Product Overview : | The microplate assay is used to determine Lactate dehydrogenase B activity (LDHB) in a sample utilizing the immunocapture technique. The specific native LDHB enzyme is immune-captured within the wells of the microplate and activity is determined by following the production of NADH catalyzed by the enzyme. The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient-37000 M-1 cm-1). The immune-capture based activity assay kit contains all the required reagents for a fast and simple measurement of LDHB activity in a whole cell extract, tissue homogenate, or serum. |
Applications : | ELISA, WB |
Storage : | All components are shipped +4ºC. Reagent dye, coupler, LDHB and NAD+ are shipped lyophilized. Before use, rehydrate according to the instructions in Section 6-Reagent Preparation and vortex each tube thoroughly to dissolve. After hydration, unused amounts of these four materials should be aliquoted and stored at -20°C, avoiding additional freeze/thaw cycles. Store all other components at 4°C. This kit is Stable for 6 months from receipt. |
◆ Recombinant Protein
LDHB-424H | Recombinant Human LDHB Protein, MYC/DDK-tagged | +Inquiry |
Ldhb-1306M | Recombinant Mouse Ldhb Protein, MYC/DDK-tagged | +Inquiry |
Ldhb-1818R | Recombinant Rat Ldhb protein, His-tagged | +Inquiry |
Ldhb-1817M | Recombinant Mouse Ldhb protein, His-tagged | +Inquiry |
LDHB-2488R | Recombinant Rhesus monkey LDHB Protein, His-tagged | +Inquiry |
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◆ Native Protein
LDH2-220H | Native Human Lactate Dehydrogenase 2 | +Inquiry |
LDH2-123H | Native Human Lactate Dehydrogenase 2 | +Inquiry |
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◆ Lysates
LDHB-4788HCL | Recombinant Human LDHB 293 Cell Lysate | +Inquiry |
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