Lactate Dehydrogenase B (LDHB) Activity Assay Kit

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Lactate Dehydrogenase B (LDHB) Activity Assay Kit

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Cat.No. : Kit-0510
Product Overview : The microplate assay is used to determine Lactate dehydrogenase B activity (LDHB) in a sample utilizing the immunocapture technique. The specific native LDHB enzyme is immune-captured within the wells of the microplate and activity is determined by following the production of NADH catalyzed by the enzyme. The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient-37000 M-1 cm-1). The immune-capture based activity assay kit contains all the required reagents for a fast and simple measurement of LDHB activity in a whole cell extract, tissue homogenate, or serum.
Applications : ELISA, WB
Storage : All components are shipped +4ºC. Reagent dye, coupler, LDHB and NAD+ are shipped lyophilized. Before use, rehydrate according to the instructions in Section 6-Reagent Preparation and vortex each tube thoroughly to dissolve. After hydration, unused amounts of these four materials should be aliquoted and stored at -20°C, avoiding additional freeze/thaw cycles. Store all other components at 4°C. This kit is Stable for 6 months from receipt.

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