LDH-Cytotoxicity Fluorometric Assay Kit
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|Product Overview :||Lactate Dehydrogenase (LDH, EC 18.104.22.168) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.|
Measurement of released LDH in culture medium.
Evaluation of cytotoxicity effect of test compounds.
|Size :||500 assays|
◆ Recombinant Protein
|LDH-0507B||Recombinant Bacillus subtilis LDH protein, His-tagged||+Inquiry|
◆ Native Protein
|LDH-227H||Native Human Lactate Dehydrogenase Total||+Inquiry|
|LDH-217R||Native Rabbit Lactate Dehydrogenase||+Inquiry|
|LDH-226H||Native Human Lactate Dehydrogenase Total||+Inquiry|
◆ Assay kits
|Kit-2088||D-Lactate Dehydrogenase Assay Kit||+Inquiry|
|Kit-2203||Lactate Dehydrogenase Activity Colorimetric Assay Kit (384-well)||+Inquiry|