LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric) is used for an easy and fast fluorometric measurement of LSD1 activity or inhibition.
The LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric) is suitable for measuring LSD1 activity/inhibition using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues. Nuclear extracts can be prepared by using your own successful method. Nuclear extracts can be used immediately or stored at -80°C for future use. Purified enzymes can be active LSD1 from recombinant proteins or isolated from cell/tissues.
Upon receipt: (1) Store LF3, LF4, LF6 and LF8 at –20°C away from light; (2) Store LF1, LF5, LF7, LF9, and 8-Well Assay Strips at 4°C away from light; (3) Store remaining components (LF2, LF10, and Adhesive Covering Film) at room temperature away from light.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: (1) Check if LF1 (10X Wash Buffer) contains salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved.