Lysozyme Activity Assay Kit (Fluorometric)

• Cat.No.: Kit-1039

Specification

• Tag: Non
• Description: Lysozyme (EC 3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the β-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. It exhibits antibacterial, antitumor and immune modulatory activities. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis and nephritis. lysozyme activity assay kit utilizes the ability of lysozyme to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 360/445 nm). This kit provides a simple, ultra-sensitive assay that can detect as low as 2 μU/ml of Lysozyme activity in a variety of biological samples.
• Applications: Detection of Lysozyme Activity in variety of samples
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Lysozyme Assay Buffer; Lysozyme Stop Buffer; Lysozyme Substrate (in DMSO); Lysozyme Positive Control (lyophilized); 4-Methylumbelliferone Standard (5 mM)
• Detection method: Fluorescence (Ex/Em = 360/445 nm)
• Features & Benefits: Rapid, and convenient; The assay can detect Lysozyme activity as low as 2 μU/ml in a variety of biological samples.