Download Datasheet
See All Malate Dehydrogenase Related Kits | Cat.No.: | Kit-2461 |
| Product Overview: | Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer's disease. The non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. |
| Storage: | -20ºC |
| Shipping: | On Ice |
| Size: | 100 tests |
| Detection method: | OD565nm |
| Compatible Sample Types: | Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). |
| Features & Benefits: | Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 65 U/L for 20 min reaction at 37°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
| Assay time: | 30 min |
| Sensitivity: | 0.5 U/L |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet