Microsome Isolation Kit
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|Product Overview :||Microsome Isolation Kit enables preparation of active microsomes in about one hour, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.|
|Size :||50 assays|
|Applications :||Convenient and fast isolation of microsomal fraction from animal tissuesAssessment of CYP-mediated drug metabolism and xenobiotic biotransformationProtein profiling of microsomal membrane proteins by SDS-PAGE and Western blot|
|Storage :||Store kit at -20°C. Read entire protocol before performing the isolation procedure.Homogenization Buffer and Storage Buffer: Buffers may be stored at -20°C or 4°C. Keep buffers on ice while in use.Protease Inhibitor Cocktail: Resuspend the lyophilized Protease Inhibitor Cocktail in 250 µl anhydrous DMSO (not provided) (500Xstock solution) and store at -20°C.|