"MAO" Related Products

Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit

Cat.No.: Kit-2221
Product Overview: Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity and tissue localization. Clonogenic studies have shown that these two isozymes have similar catalytic characteristics, yet their amino acid sequences are different. MAO-A favors Serotonin, Norerpinephrine and Dopamine as substrates, while phenylethylamine and benzylamine are MAO-B preferred substrates. MAO-A and MAO-B are mitochondrial-bound enzymes that are ubiquitously expressed throughout the brain and other tissues. Imbalance of MAOs levels has been associated with schizophrenia, depression, attention deficit and other disorders. MAO-A has been implicated in panic, anxiety and depression, whereas MAO-B defects result in Alzheimer’s and Parkinson’s diseases. Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) assay is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAO-B, respectively. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of the MAO substrate. The assay can detect as little as 5 μU of MAO enzymatic activity.
Applications: Measurement of Total MAO, MAO-A and MAO-B activities in various biological samples.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: MAO Assay Buffer; OxiRed Probe (in DMSO); MAO Substrate (Lyophilized); Developer (Lyophilized); Positive Control (MAO-A Enzyme) (Lyophilized) (Lyophilized); MAO-A Inhibitor, Clorgyline (Lyophilized); MAO-B Inhibitor, Selegiline (Lyophilized); H2O2 Standard (0.88 M)
Detection method: Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits: Rapid & Convenient;
The assay can detect as little as 5 μU of MAO enzymatic activity.
Includes MAO-A specific Inhibitor Clorgyline, MAO-B specific Inhibitor Selegiline & MAO-A Enzyme as Positive Control

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.