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Mouse embryonic fibroblast cell line NIH 3T3 Whole cell Lysate

Cat.No. : NIH3T3-051WCY
Product Overview : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell Lysate
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Description : NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated at the New York University School of Medicine Department of Pathology in 1962.
Source : Embryo
Species : Mouse
Applications : IP, WB
Stability : The lysate is stable for at least one year from date of shipping when stored at -80 centigrade. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20 centigrade for long term storage.
Storage : Store the lysate at -80 centigrade and avoid repeated freeze / thaw cycles.
Concentration : 1 vial of 100 μg whole cell lysate in RIPA buffer.
1 vial of 250 μL 2 x SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH 6.8, 10 % Glycerol, 0.002 % Bromphenol blue, 100 mM DTT)
Storage Buffer : Modified RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 x Proteinase inhibitor cocktail mix (Sigma) and 1 mM Na3VO4)
Shipping : Shipped on dry ice.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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