"NAD/NADH" Related Products

NAD+/NADH Colorimetric Assay Kit (384-well)

Cat.No.: Kit-1048
Description: Nicotinamide Adenine Dinucleotide (NAD) is a co-enzyme indispensible for the activity of multiple catalytic proteins. NAD+ and NADH, its reduced form, participate in many biological redox reactions. NAD+ is mostly associated with redox enzymes required for catabolic reaction and energy production. NADH/NAD Quantification Kit provides a convenient tool for a sensitive detection of NADH, NAD and their ratio. There is no requirement to purify NADH or NAD from samples. The NADH selective developer develops color while reacting with NADH and can be detected at OD 450 nm. The total NAD can be easily quantified by comparing with the provided NADH standard. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format.The kit can detect as low as 0.04 pmol of NADH in a 384 well assay plate. The reaction specifically detects NADH and NAD, but not NADP nor NADPH.
Applications: NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio
Storage: -20°C
Shipping: Gel Pack
Size: 400 assays
Kit Components: NADH/NAD Extraction Buffer; NAD Cycling Buffer; NAD Cycling Enzyme Mix; NADH Developer; Stop Solution; NADH Standard
Target Species: Mammalian
Detection method: Absorbance (450 nm)
Features & Benefits: Simple procedure; takes ~2 hours;
Fast and convenient;
Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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