NADPH Fluorimetric Assay Kit (Red)

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NADPH Fluorimetric Assay Kit (Red)

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Cat.No. : Kit-2050
Product Overview : This Fluorimetric NADPH Assay Kit provides a convenient method for the detection of NADPH. The enzymes in the system specifically recognize NADPH in an enzyme recycling reaction. In addition, this assay has very low background since it is run in the red visible range that significantly reduces the interference from biological samples.
Size : 400 assays in 96-well plates
Applications : The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.This kit has been used for screening enzyme activities that use NADP/NADPH as a cofactor. It has also been used for the sensitive detection of NADPH in cell-based assays. Compared to the other commercial kits, this assay has higher signal/background ratio.
Storage : Keep Component A in freezer (-20°C) and avoid exposure to light; Component C at -20°C; Component B &G at 4°C.

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