Native Bovine NEFH Protein
Cat.No. : | NEFH-181B |
Product Overview : | Native Bovine NEFH Protein |
- Specification
- Gene Information
- Related Products
Description : | Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, d are composed predominantly of four major proteins called NF-L, NF-M, NF-H and α-internexin. NF-H is the neurofilament heavy or high molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kDa in the heavily phosphorylated axonal form. The molecule has an unusual and interesting region consisting of multiple Lysine-Serine-Proline peptides, about 40 of these in human. These peptide repeats are heavily phosphorylated on the Serine residues in axons. Enzymatic removal of these phosphate groups will increase the SDS-PAGE mobility to about 160kDa, likely due to protein conformational changes due to the removal of charge. Even the non-phosphorylated form runs aberrantly on SDS-PAGE, as the real molecular weight of NF-H is about 110kDa, with some variation in different species. This is likely due to an unusually high content of charged amino acids. Non-phosphorylated forms of NF-H are found in dendrites and perikarya and early in development, but the majority of NF-H in the adult is this heavily phosphorylated axonal form. Our preparation was isolated from cow spinal cord using a modification of the method of Leung and Liem, which purifies out the heavily phosphorylated axonal form, often referred to as pNF-H. The HGNC name for this protein is NEFH. |
Species : | Bovine |
Molecular Mass : | ~220kDa by SDS-PAGE |
Applications : | ELISA, western blotting standard |
Storage : | Store at -20 centigrade |
Concentration : | 1 mg/mL |
Storage Buffer : | 6M Urea |
Gene Name : | NEFH neurofilament heavy chain [ Bos taurus (cattle) ] |
Official Symbol : | NEFH |
Synonyms : | NEFH; neurofilament heavy chain; NF-H; LOW QUALITY PROTEIN: neurofilament heavy polypeptide; 200 kDa neurofilament protein; Neurofilament triplet H protein; neurofilament heavy polypeptide |
Gene ID : | 528842 |
mRNA Refseq : | XM_005218112 |
Protein Refseq : | XP_005218169 |
UniProt ID : | F1MSQ6 |
Products Types
◆ Recombinant Protein | ||
NEFH-1496H | Recombinant Human NEFH Protein, His (Fc)-Avi-tagged | +Inquiry |
Nefh-4362M | Recombinant Mouse Nefh Protein, Myc/DDK-tagged | +Inquiry |
Nefh-1821R | Recombinant Rat Nefh Protein, His-tagged | +Inquiry |
NEFH-3270H | Recombinant Human NEFH protein, His-SUMO & Myc-tagged | +Inquiry |
NEFH-1254H | Recombinant Human NEFH, His-tagged | +Inquiry |
◆ Native Protein | ||
NEFH-180B | Native bovine NEFH | +Inquiry |
NEFH-01P | Native Pig NEFH Protein | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket