Native Bovine NEFM

• Cat.No.: NEFM-1520B
• Product Overview: Ultra Pure NeuroFilament Protein having a Molecular mass of 160 kDa produced from Bovine Spinal Cord.

Specification

• Species: Bovine
• Source: bovine Spinal Cord
• Tag: Non
• Description: Neurofilaments are type IV intermediate filament heteropolymers that are composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber and may also have a role in intracellular transport to axons and dendrites. NeuroFilament 160kDa is a medium neurofilament protein, which is commonly used as a biomarker of neuronal damage.
• Form: The protein was lyophilized from a 1mg/ml solution containing 10mM sodium phosphate, pH-7.5, 2mM DTT, 6M urea and 1mM EDTA.Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Molecular Mass: 103,210 Da
• Purity: Greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization.
• Notes: Small volumes of NEFM native protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Reconstitution: It is recommended to reconstitute the lyophilized NEFM in sterile 18M Ω-cm H2O.
• Warning: It is recommended to reconstitute the lyophilized NEFM in sterile 18M Omega -cm H2O.

Gene Information

• Gene Name: NEFM neurofilament, medium polypeptide [ Bos taurus ]
• Official Symbol: NEFM
• Synonyms: NEF3; NF-M; neurofilament medium polypeptide; neurofilament 3; neurofilament M subunit; 160 kDa neurofilament protein; neurofilament triplet M protein; major neuronal intermediate filament subunit
• Gene ID: 281347
• mRNA Refseq: NM_001244145
• Protein Refseq: NP_001231074
• Chromosome Location: chromosome: 8
• Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem
• Function: structural molecule activity