Native Clostridium histolyticum Clostripain, Sequencing Grade
| Cat.No. : | Clostripain-02C |
| Product Overview : | Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. |
- Specification
- Gene Information
- Related Products
- Download
| Species : | Clostridium histolyticum |
| Description : | Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory. |
| Form : | A dialyzed, lyophilized, pre-activated powder. |
| Bio-activity : | ≥50 units per mg dry weight |
| CAS # : | 9028-00-6 |
| Molecular Mass : | 53.0 kDa Light chain: 12.5 kDa Heavy chain: 45 kDa |
| Specificity : | Clostripain selectively hydrolyzes arginyl bonds and lysyl bonds at a lower rate. It can also act as a transpeptidase with maximal activity at pH 7.6-9.0. |
| Extinction Coefficient : | 87,890 cm-1 M-1 (Theoretical) E1%,280= 16.57 (Theoretical) |
| Isoelectric point : | 4.8-4.9 |
| Optimal pH : | 7.4-7.8 (activity against a-benzoyl-arginine ethyl ester) |
| Activators : | Sulfhydryl requirement: dithiothreitol, cysteine, or other reducing agents Calcium ion is essential Reducing agents |
| Inhibitors : | EDTA Oxidizing agents Sulfhydryl reagents (such as TLCK) Co2+, Cu2+, Cd2+, and heavy metal ions Citrate, borate and Tris anions partially inhibit |
| Unit Definition : | One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25 centigrade, pH 7.6, in the presence of dithiothreitol. |
| Applications : | Peptide mapping Sequence analysis Cell isolation Hydrolysis/condensation of amide bonds Peptide synthesis |
| Storage : | Store at 2-8 centigrade. |
| Official Symbol | Clostripain |
| Synonyms | Clostripain; Endoproteinase-Arg-C; CAS 9028-00-6; EC: 3.4.22.8 |
| UniProt ID | P09870 |
| ◆ Native Proteins | ||
| Clostripain-02C | Native Clostridium histolyticum Clostripain, Sequencing Grade | +Inquiry |
| Clostripain-01C | Native Clostridium histolyticum Clostripain | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews (0)
- Q&As (0)
Ask a Question for All Clostripain Products
Required fields are marked with *
My Review for All Clostripain Products
Required fields are marked with *
Recently Viewed
