Native Diphtheria Toxin CRM197, Mutant [Glu52]


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Native Diphtheria Toxin CRM197, Mutant [Glu52]

CRM197 Related Products

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Cat.No. : CRM197-190
Product Overview : CRM197 was expressed in Corynebacterium diphtheriae, and purified by state-of-art protein purification method such as centrifugation, amonium sulfate precipitation and chromatography, then lyophilized after addition of sucrose. Moisture: <3% by Karl-Fisher
Description : The Glu52 diptheria toxin, also known as CRM197 (Cross-reactive materials 197), is a nontoxic mutant of diphtheria toxin, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity.
Source : Corynebacterium diphtheriae
Species : Corynebacterium diphtheriae
Form : Lyophilized powder (liquid also could be provided)
Molecular Mass : ~58 kDa
Sterility : Inoculation/membrane filtration
Endotoxin : <10 EU/μg of protein by LAL method.
Purity : > 90% by SDS-PAGE and HPLC
Storage : Store at -20 centigrade.
Concentration : 1~20mg/ml(aq) by BCA or Lowry
Reconstitution : Before lyophilization, CRM197(aq) was about 5mg/ml in sterile water with 5% sucrose. Vials containing appoximately 5mL of CRM197(aq) were lyophilized. The lyophilized powder can be reconstituted with water or sodium phosphate buffer. We could provide liquid or lyophilized powder.
Figure :

Fig 1 A. Purity analysis of CRM197 by SDS PAGE (10%); B. Identity test of CRM197 by mass spectrometry, Mascot score histogram(top), sequence coverage (bottom); C. Identity test of CRM197 by western blotting; D. Purity analysis of CRM197 by HPLC.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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