Native Diphtheria Toxin CRM197, Mutant [Glu52]
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|Product Overview :||CRM197 was expressed in Corynebacterium diphtheriae, and purified by state-of-art protein purification method such as centrifugation, amonium sulfate precipitation and chromatography, then lyophilized after addition of sucrose. Moisture: <3% by Karl-Fisher|
|Description :||The Glu52 diptheria toxin, also known as CRM197 (Cross-reactive materials 197), is a nontoxic mutant of diphtheria toxin, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity.|
|Source :||Corynebacterium diphtheriae|
|Species :||Corynebacterium diphtheriae|
|Form :||Lyophilized powder (liquid also could be provided)|
|Molecular Mass :||~58 kDa|
|Sterility :||Inoculation/membrane filtration|
|Endotoxin :||<10 EU/μg of protein by LAL method.|
|Purity :||> 90% by SDS-PAGE and HPLC|
|Storage :||Store at -20 centigrade.|
|Concentration :||1~20mg/ml(aq) by BCA or Lowry|
|Reconstitution :||Before lyophilization, CRM197(aq) was about 5mg/ml in sterile water with 5% sucrose. Vials containing appoximately 5mL of CRM197(aq) were lyophilized. The lyophilized powder can be reconstituted with water or sodium phosphate buffer. We could provide liquid or lyophilized powder.|
Fig 1 A. Purity analysis of CRM197 by SDS PAGE (10%); B. Identity test of CRM197 by mass spectrometry, Mascot score histogram(top), sequence coverage (bottom); C. Identity test of CRM197 by western blotting; D. Purity analysis of CRM197 by HPLC.