Recombinant Diphtheria Toxin Mutant CRM197, Low Endotoxin

• Cat.No.: COR-126
• Product Overview: Low Endotoxin Recombinant Diphtheria Toxin Mutant CRM197 was expressed in E. coli.

Specification

• Source: E.coli
• Tag: Non
• Protein Length: 535 aa
• Form: Lyophilized from a 0.2 um filtered concentrated solution in 20mM PB, 0.025% Tween-80, pH7.4.
• Molecular Mass: Approximately 58.4kDa, a single non-glycosylated polypeptide chain containing 535 amino acids.
• AASequence: GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKEFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKTQPFLHDGYAVSWNTVEDSIIRTGFQGESGHDIKITAENTPLPIAGVLLPTIPGKLDVNKSKTHISVNGRKIRMRCRAIDGDVTFCRPKSPVYVGNGVHANLHVAFHRSSSEKIHSNEISSDSIGVLGYQKTVDHTKVNSKLSLFFEIKS
• Endotoxin: Less than 0.01EU/ug of rCRM197 as determined by LAL method
• Purity: >96% by SDS-PAGE or HPLC
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.
• Reconstitution: This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.