|Product Overview:||This product is the unconjugated Griffonia (Bandeiraea) Simplicifolia Lectin I and has sugar specificity against Galactose, N-Acetylgalactosamine.|
|Description:||GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. This GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.|
|Bio-activity:||Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine.
Carbohydrate-Binding Specificity: Galactose, N-Acetylgalactosamine.
|Molecular Mass:||114 kDa|
|Applications:||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology|
|Storage:||Refrigerate in the dark|
|Reconstitution:||Although many buffers can be employed for reconstituting and diluting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+. For preserving solutions stored at 4 centigrade, add 0.08% sodium azide to the buffer.|
|Synonyms:||Lectin; GSL I; BSL I|