| Description : |
This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. |
| Source : |
Griffonia Simplicifolia |
| Species : |
Griffonia Simplicifolia |
| Bio-activity : |
Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine |
| Molecular Mass : |
113 kDa |
| Applications : |
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Storage : |
Refrigerate; for long term storage, aliquots may be stored frozen. |
| Reconstitution : |
Reconstitute biotinylated lectin by addition 1ml of water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM Ca2+. For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer |
| Synonyms : |
Lectin; GSL II; BSL II |
| UniProt ID : |
Q41263 |
