Native Kidney bean Phaseolus vulgaris Lectin (PHA-E), Ferritin-Conjugated

• Cat.No.: PHA-E-279K
• Product Overview: Native Kidney bean Phaseolus vulgaris Lectin (PHA-E) - Ferritin
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: Complex N-Glycans
  Inhibiting or Eluting Sugar: Bovine Thyroglobulin, Acetic Acid
  Divalent Ions: Ca++, Mn++
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous

Specification

• Species: Kidney bean
• Source: Kidney bean
• Description: Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. PHA-E binds di-galactosylated and bisected N-glycan. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins. Phaseolus vulgaris Lectin (PHA-E) is conjugated to ferritin, that has a molecular weight of 474,000 g/mol (all 24 subunits combined). Ferritin-conjugated lectins can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins. These conjugates can be viewed via electron microscopy.
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL

Gene Information

• Official Symbol: PHA-E
• Synonyms: Phaseolus vulgaris Lectin; PHA-E)