| Species : |
Ocean Fish |
| Tag : |
Non |
| Description : |
Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6. |
| Form : |
Liquid (sterile filtered), 0.5 M Acetic Acid / Lyophilized powder. |
| Purity : |
This product has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with Rockland’s anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA). |
| Applications : |
ELISA : 1:10,000
Western Blot : 1:1000
Immunohistochemistry: 1:100-1:500
Other Dilution: User Optimized |
| Storage : |
Store vial at 2-30°C. |
| Concentration : |
1.0 mg/mL by dry weight |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
