Native Porcine Arp 2/3 complex Protein

• Cat.No.: Arp 2/3 complex-856P
• Product Overview: Native Porcine Arp 2/3 complex was purified from Porcine brain.

Specification

• Species: Porcine
• Source: Porcine Brain
• Tag: Non
• Description: The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller (Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins, namely p16, p20, p21, p34, and p40 (Synonyms: ARPC-5, -4, -3, -2, and -1).
  Arp 2/3 is a constitutively inactive complex. Nucleation is efficiently activated in vitro by binding of the VCA-domains of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous).
• Form: 150mM KCl, 20mM Trizma pH 7.5, 1mM DTT, 0.2mM ATP, 0.5mM EDTA, 1mM MgCl2 and 5% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1 mg/ml solution.
• Molecular Mass: 224kDa
• Purity: >98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
• Storage: Arp2/3 complex is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect. Avoid repeated freeze / thaw cycles.
• Shipping: At ambient temperature. Upon delivery store at -70 centigrade.