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Native Porcine GSN Protein

Cat.No.: GSN-874P
Product Overview: Native Porcine GSN was purified from Porcine smooth muscle.
Description: Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin regulatory protein, possessing three different actin modulating activities: severing of F-actin, nucleation of actin polymerization and capping of filaments barbed end.
Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and two binding sites for PIP2.
Source: Porcine smooth muscle
Species: Porcine
Form: 10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and 1% disaccharides, when reconstituted with 250µl ultrapure water to obtain a 1.0 mg/ml solution.
Bio-activity: Fragmentation activity: The fragmentation activity of gelsolin was determined by Ostwald viscometry at 25 centigrade. The specific viscosity of F-actin (1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100. The gelsolin stock solution was used as described below containing 1% disaccharides.
F-actin containing 100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition of gelsolin.
Molecular Mass: 80kDa
Purity: >90% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Storage: Gelsolin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 5 days. Storage in glycerol at -20 centigrade results in a significant decrase of the activity, and has to determined individually.
Shipping: At ambient temperature. Upon delivery store at -70 centigrade.
Gene Name: GSN gelsolin [ Sus scrofa ]
Official Symbol: GSN
Synonyms: gelsolin
Gene ID: 396874

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