| Description : |
Cytoplasmic gelsolin has a molecular mass of 80kDa and consists of six homologous subdomains (S1-S6). Gelsolin is a Ca++-dependent lipid binding actin regulatory protein, possessing three different actin modulating activities: severing of F-actin, nucleation of actin polymerization and capping of filaments barbed end.
Gelsolin has three actin binding sites, two high affinity Ca++ binding sites and two binding sites for PIP2. |
| Source : |
Porcine smooth muscle |
| Species : |
Porcine |
| Form : |
10mM Imidazole pH 7.0, 0.2mM DTT, 0.2mM EGTA, 2mM NaN3 and 1% disaccharides, when reconstituted with 250µl ultrapure water to obtain a 1.0 mg/ml solution. |
| Bio-activity : |
Fragmentation activity: The fragmentation activity of gelsolin was determined by Ostwald viscometry at 25 centigrade. The specific viscosity of F-actin (1mg/ml) is reduced by ~70% after addition of gelsolin at a molar ratio of 1:100. The gelsolin stock solution was used as described below containing 1% disaccharides.
F-actin containing 100mM KCl, 10mM Imidazole pH 7.4, 1mM ATP was mixed with 0.2mM CaCl2 (final concentration) prior to the addition of gelsolin. |
| Molecular Mass : |
80kDa |
| Purity : |
>90% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
| Storage : |
Gelsolin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 5 days. Storage in glycerol at -20 centigrade results in a significant decrase of the activity, and has to determined individually. |
| Shipping : |
At ambient temperature. Upon delivery store at -70 centigrade. |
