Native Rabbit Myosin II Protein


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Native Rabbit Myosin II Protein

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Cat.No. : MYH-11R
Product Overview : Myosin II protein has been purified from rabbit skeletal muscle. The full length myosin II protein has been purified with its essential light chains (ELC) and regulatory light chains (RLC)
Source : Rabbit skeletal muscle
Species : Rabbit
Form : Lyophilized from 100 mM Imidizole pH 7.5, 2.4 M KCl, 0.2 mM DTT, 5% sucrose and 1% dextran.
Bio-activity : The biological activity of rabbit myosin II is determined from its rate of F-actin activated ATP hydrolysis. A standard biological assay for monitoring ATP hydrolysis by myosin consists of an in vitro F-actin ATPase assay. Stringent quality control ensures that in the presence of F-actin, rabbit myosin will have a minimum hydrolysis rate 10 fold greater than in the absence of F-actin, which is comparable to published results.
Molecular Mass : The myosin heavy chain (approx. 200 kDa), arrowheads indicate the RLC (approx. 20 kDa) and two ELC isoforms (approx. 25 and 17 kDa).
Purity : 95%
Stability : The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for 1 year.
Reconstitution : Reconstituted in nanopure water.
Synonyms : Myosin II; light chains
Figure :

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