|Product Overview:||R-PE is a phycobiliprotein purified from Febico’s proprietary red algae. It is made up of (αβ) 6γ subunit structure.
ABSORPTION MAXIMUM: 566 nm
EMISSION MAXIMUM: 575 nm
EXTINCTION COEFFICIENT: 1.96 X 106 cm^-1 M^-1
QUANTUM YIELD: 0.84
|Description:||R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields.|
|Molecular Mass:||240 kDa|
|Purity:||A566/A280 ≥ 5.5
|Applications:||1. Can be cross linked or conjugated to other molecules to make fluorescent probes.
2. Useful as a fluorescence-based indicator for the presence of cyanobacteria.
3. Can be used for a variety of immunofluorescence applications.
4. A useful fluoroprobe in various electrophoretic procedures.
|Usage:||For Research Use Only! Not to be used in humans|
|Stability:||The protein is very stable and can be stored in this buffer for many years.|
|Storage:||Store R-PE in dark at ~ 2-8 centigrade. Do not freeze|
|Storage Buffer:||100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.|
|Reconstitution:||Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4 centigrade. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed or desalted in columns prior to use, to remove any remaining ammonium sulphate.|
|Handling:||Centrifuge the vial prior to opening.|
|Synonyms:||RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin|