Native Red Fluorescent Protein
Cat.No. : | R-PE-004R |
Product Overview : | R-PE is a phycobiliprotein purified from Febico’s proprietary red algae. It is made up of (αβ) 6γ subunit structure. ABSORPTION MAXIMUM: 566 nm EMISSION MAXIMUM: 575 nm EXTINCTION COEFFICIENT: 1.96 X 106 cm^-1 M^-1 QUANTUM YIELD: 0.84 |
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Description : | R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. |
Source : | Red algae |
Species : | Red algae |
Form : | Suspension |
Molecular Mass : | 240 kDa |
Purity : | A566/A280 ≥ 5.5 A566/A498 ≤1.5 |
Applications : | 1. Can be cross linked or conjugated to other molecules to make fluorescent probes. 2. Useful as a fluorescence-based indicator for the presence of cyanobacteria. 3. Can be used for a variety of immunofluorescence applications. 4. A useful fluoroprobe in various electrophoretic procedures. |
Usage : | For Research Use Only! Not to be used in humans |
Stability : | The protein is very stable and can be stored in this buffer for many years. |
Storage : | Store R-PE in dark at ~ 2-8 centigrade. Do not freeze |
Storage Buffer : | 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide. |
Reconstitution : | Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4 centigrade. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed or desalted in columns prior to use, to remove any remaining ammonium sulphate. |
Handling : | Centrifuge the vial prior to opening. |
Synonyms : | RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin |
Synonyms : | RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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