| Description : |
The major regulatory protein of SV40 virus, responsible for virion assembly, viral and cellular transcriptional regulation, viral DNA replication, and alteration of the cell cycle. |
| Source : |
Simian virus 40 |
| Species : |
Insect Cells |
| Form : |
10 mM Tris-HCl (pH 8.0)100 mM NaCl1.0 mM dithiothreitol1.0 mM EDTA50% (v/v) glycerol |
| Bio-activity : |
1 µl of protein is required to show T-antigen dependence of SV40 DNA replication in vitro. |
| Applications : |
1. Simian virus (SV40) DNA is the best model for mammalian chromosomal DNA replication in vitro.2. Examining fidelity and biochemistry of DNA replication in humans.3. Identifying mode of action and mechanisms leading to cell transformation in vitro and tumor formation in vivo.4. Regulating gene expression in eukaryotic cells.5. Studying the inhibition of DNA replication by mutagenesis.6. Determining risks posed by human carcinogens Comparing replication of normal and malignant cells Investigating the cytotoxic effects of drugs.7. Investigating apoptosis. |
| Quality Control Test : |
All preparations are assayed for stimulation of DNA synthesis in a T antigen-dependent DNA replication assay;Typical preparations are greater than 90% pure as judged by SDS polyacrylamide gel electrophoresis |
| Storage : |
Store at -20 centigrade |
| Concentration : |
1.08 µg / µl |
