|Product Overview:||Native Turkey VCL tail was purified from Turkey Smooth muscle.|
|Description:||Vinculin tail is obtained by limited cleavage with protease V8 from S. aureus of natively prepared smooth muscle vinculin from turkey gizzard. The C-terminal tail region (851-1066) binds to F-actin and was shown to bundle actin filaments in vitro (Hüttelmaier et al., 1997).
Since vinculin is autoinhibited by a head/tail interaction, the tail region is used to characterize the actin interactions of vinculin.
|Source:||Turkey Smooth muscle|
|Form:||PBS, 0.5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1mg/ml solution.|
|Purity:||>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.|
|Storage:||Vinculin tail is stored at –70 centigrade upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 2-3 weeks.
This product can also be stored as a glycerol stock at -20 centigrade. Avoid repeated freeze / thaw cycles.
|Shipping:||At ambient temperature. Upon delivery store at -70 centigrade.|