"VCL tail" Related Products

Native Turkey VCL tail Protein

Cat.No.: VCL tail-900T
Product Overview: Native Turkey VCL tail was purified from Turkey Smooth muscle.
Description: Vinculin tail is obtained by limited cleavage with protease V8 from S. aureus of natively prepared smooth muscle vinculin from turkey gizzard. The C-terminal tail region (851-1066) binds to F-actin and was shown to bundle actin filaments in vitro (Hüttelmaier et al., 1997).
Since vinculin is autoinhibited by a head/tail interaction, the tail region is used to characterize the actin interactions of vinculin.
Source: Turkey Smooth muscle
Species: Turkey
Form: PBS, 0.5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1mg/ml solution.
Molecular Mass: 29/27kDa
Purity: >99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Storage: Vinculin tail is stored at –70 centigrade upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 2-3 weeks.
This product can also be stored as a glycerol stock at -20 centigrade. Avoid repeated freeze / thaw cycles.
Shipping: At ambient temperature. Upon delivery store at -70 centigrade.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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