Native Turkey VCL tail Protein

• Cat.No.: VCL tail-900T
• Product Overview: Native Turkey VCL tail was purified from Turkey Smooth muscle.

Specification

• Description: Vinculin tail is obtained by limited cleavage with protease V8 from S. aureus of natively prepared smooth muscle vinculin from turkey gizzard. The C-terminal tail region (851-1066) binds to F-actin and was shown to bundle actin filaments in vitro (Hüttelmaier et al., 1997).
  Since vinculin is autoinhibited by a head/tail interaction, the tail region is used to characterize the actin interactions of vinculin.
• Source: Turkey Smooth muscle
• Species: Turkey
• Form: PBS, 0.5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1mg/ml solution.
• Molecular Mass: 29/27kDa
• Purity: >99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
• Storage: Vinculin tail is stored at –70 centigrade upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 2-3 weeks.
  This product can also be stored as a glycerol stock at -20 centigrade. Avoid repeated freeze / thaw cycles.
• Shipping: At ambient temperature. Upon delivery store at -70 centigrade.