|Product Overview:||This product is the Agarose bound Ulex Europaeus Agglutinin I and has sugar specificity against fucose and arabinose.|
|Description:||Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.|
|Form:||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08 % sodium azide,|
|Bio-activity:||Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose
Carbohydrate-Binding Specificity: Fucose and Arabinose
|Molecular Mass:||63 kDa|
|Applications:||Glycobiology, Affinity Chromatography|
|Usage:||1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin.
2, Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution. Alternatively, 100 mM L-fucose in buffered saline can be used.
3, After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
|Storage:||Refrigerate. DO NOT FREEZE.|
|Concentration:||2 mg/ml of settled gel|
|Synonyms:||Lectin; UEA I|
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