|Product Overview:||This product is the ulex europaeus lectin which againsts fucose blood group H. Affinity purified UEA I is conjugated with Fluorescein-5- isothiocyanate (FITC-isomer 1) and purified on Gel filtration column. A max. = 490 nm, E max =520 nm, Ratio of A490/A280= 1.0-1.4.|
|Description:||The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.Inhibiting/Eluting sugars: 50-100 mM L-fucoseCarbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R|
|Form:||10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide.|
Immunofluorescence 1:50-1:200. The optimum dilution should be determined by the individual lab.
|Storage:||Store at 2-8centigrade.|
|Concentration:||1 mg/ml (Please refer to the vial label for the specific concentration)|
|Synonyms:||UAE, Ulex europaeus, UAE I Lectin, Fucose lectin, UAE I, Ulex europaeus lectin, UAE Lectin|