Ni-NTA Agarose

• Cat.No.: Ni-NTA-001A
• Product Overview: Ni-NTA is designed for high quality purification of 6xhis-tagged recombinant proteins from bacteria, insect and mammalian cells. Ni-NTA agarose beads are widely used for protein purification due to its high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.

Specification

• Tag: Non
• Cat.no: Ni-NTA-001A
• Characteristics: NTA binds Ni2+ ions by four coordination sites. The Ni-NTA Agarose exhibits high affinity and selectivity for 6xhis-tagged recombinant fusion proteins, which can be purified under native, denaturing, or hybrid conditions using the Ni-NTA Agarose. Proteins bound to the resin are eluted with low pH buffer or by competition with imidazole or histidine.
• Form: It is provided as a 50% slurry in 30% ethanol.
• Size: 10 mL, 25 mL, 100 mL
• Matrix: 4% cross-linked agarose
• Average particle size: 45-165 μm
• Ligand: Nitrilotriacetic acid (NTA)
• Dynamic binding capacity: 5-10 mg of protein per ml of resin
• Recommended flow rate: 30 ml/h
• Recommended column height: 5‐20cm
• Chemical stability: Stable to all solutions commonly used in gel filtration including reducing agents
• Physical stability: Ni-NTA resin is guaranteed stable for 6 months when properly stored.
• pH working range: 3-13
• pH CIP range: 2-14
• Temperature Stability: 4‐40 °C
• Warning: Avoid using protease inhibitors or other additives that contain chelators, such as EDTA, or strong reducing agents, such as DTT, which will disrupt the function of nikel resin.