||Nitrate/Nitrite Colorimetric Assay Kit (LDH method) is a measurement for detecting low levels of nitrite.
||Nitric Oxide (NO) is synthesized in biological systems by the enzyme Nitric Oxide Synthase (NOS). NOS is a remarkably complex enzyme which acts on molecular oxygen, arginine, and NADPH to produce NO, citrulline, and NADP+. This process requires five additional cofactors (FMN, FAD, Heme, calmodulin, and tetrahydrobiopterin) and two divalent cations (calcium and heme iron). Three distinct isoforms of NOS have been identified.
||For research use only (RUO)
||This kit will perform as specified if stored at -20°C and used before the expiration date indicated on the outside of the box.Component StorageNitrate/Nitrite Assay Buffer 4°CNitrate Reductase (LDH method) -20°CLactate Dehydrogenase Cofactor Preparation -20°CNitrate Standard 4°C or Room TemperatureLactate Dehydrogenase -20°CGriess Reagent R1 4°CGriess Reagent R2 4°C96-Well Plate (Colorimetric Assay) Room Temperature96-Well Cover Sheets Room Temperature
||Nitrate/Nitrite Assay Buffer 1 vialNitrate Reductase (LDH method) 1 vialLactate Dehydrogenase Cofactor Preparation 1 vialNitrate Standard 1 vialLactate Dehydrogenase 1 vialGriess Reagent R1 1 vialGriess Reagent R2 1 vial96-Well Plate (Colorimetric Assay) 1 plate96-Well Cover Sheets 1 cover