Nitrile reductase (NRED)

• Cat.No.: NRED-26

Specification

• Description: NRED can catalyze the reduction of nitrile group to amine. In the process of catalysis, NADPH is needed as hydrogen transporter.
• Instructions and Precautions: The following reaction system could be used for reference: 1-10 mg/mL Substrate, 10 mg/mL Enzyme, 10% (v/v) Organic cosolvent, 100 mM Phosphate buffer (pH 7.0), 0.2 mg/mL NADP+, 10 mg/mL Glucose, 2 mg/mL Glucose dehydrogenase (GDH) Incubate the reactions at a constant temperature shaker (for example, 30 centigrade, 150 rpm) in for 24~48 hours. Centrifuge each mixture to precipitate protein, them the supernatant can be used for TLC, HPLC or GC analysis. Note: Organic solvent (such as DMSO, methanol, acetonitrile, IPA, etc.) is optional, used for insoluble substrates.

Gene Information

• Official Symbol: NRED
• Synonyms: Nitrile reductase; NRED