Nucleosome Remodeling Assay Substrate
Cat.No. : | NUC-058 |
Product Overview : | Mononucleosomes assembled from recombinant human histones expressed in E. coli. |
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Description : | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A-Cy5, H2B, H3.2 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped with a 207 base-pair 5' Cy3-conjugated DNA sequence that includes the Widom 601 nucleosome positioning element, with an added GATC motif. H2A has a Thr to Cys substitution at residue 120, the cysteine where Cy5 is conjugated. H3.2 has a Cys to Ala substitution at residue 110. |
Source : | E. coli |
Species : | Human |
Tag : | N/A |
Form : | Purified recombinant mononucleosomes, 50 µg (DNA + protein weight) in 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. |
Molecular Mass : | 242,900 Da |
Applications : | This product is a template for nucleosome remodeling enzymes (e.g. ACF Remodeling Enzyme Complex) using Cy3/Cy5 FRET, or using the restriction enzyme DpnII to determine accessibility of GATC which is masked in its native configuration (prior to remodeling). |
Storage : | Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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