Nucleosome Remodeling Assay Substrate ST601-GATC1

• Cat.No.: NUC-059
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by provided 217 base pair DNA sequence that includes the Widom 601 sequence with an added GATC.
• Form: Purified recombinant mononucleosomes in 10mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 242,900 Da
• Applications: This product is a substrate for chromatin remodeling assays (e.g. ACF Remodeling Enzyme Complex) using restriction enzymes to determine accessibility of GATC subsequent to chromatin remodeling.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.